Table 1.
shRNAs and primers used in the study
| Primer | 5′ to 3′ |
|---|---|
| sh-KDM6A-1-F | CCGG GCAGCACGAATTAAGTATTTA CTCGAG TAAATACTTAATTCGTGCTGC TTTTTG |
| sh-KDM6A-1-R | AATTCAAAAA GCAGCACGAATTAAGTATTTA CTCGAG TAAATACTTAATTCGTGCTGC |
| sh-KDM6A-2-F | CCGG GATGCAAGTCTATGACCAATT CTCGAG AATTGGTCATAGACTTGCATC TTTTTG |
| sh-KDM6A-2-R | AATTCAAAAA GATGCAAGTCTATGACCAATT CTCGAG AATTGGTCATAGACTTGCATC |
| sh-SND1-1-F | CCGG GCTGATGATGCAGACGAATTT CTCGAG AAATTCGTCTGCATCATCAGC TTTTTG |
| sh-SND1-1-R | AATTCAAAAA GCTGATGATGCAGACGAATTT CTCGAG AAATTCGTCTGCATCATCAGC |
| sh-SND1-2-F | CCGG GAAGGCATGAGAGCTAATAAT CTCGAG ATTATTAGCTCTCATGCCTTC TTTTTG |
| sh-SND1-2-R | AATTCAAAAA GAAGGCATGAGAGCTAATAAT CTCGAG ATTATTAGCTCTCATGCCTTC |
| KDM6A H1146A-F | AGGTgcTCAGGAAAATAACAACTTCTGTTCAGTTAACATAAATAT |
| KDM6A H1146A-R | CCTGAgcACCTGGTGTTCTGCTCCCTGGAACT |
| KDM6AH101D & P110S-F | GGTGACTTCAACCTCTTATTGGAAGATTATTCAAAAGCATTA |
| KDM6AH101D & P110S-R | TAATGCTTTTGAATAATCTTCCAATAAGAGGTTGAAGTCACC |
| KDM6A L259M-F | CTGTAGATCTCATGGGAGATAAAGCCACCA |
| KDM6A L259M-R | TCTCCCATGAGATCTACAGTGTGATGCATCCA |
| KDM6A N1156T-F | AGGAAACTAACAACTTCTGTTCAGTTAACATAAATATT |
| KDM6A N1156T-R | TGTTAGTTTCCTGATGACCTGGTGTTCTGCTCC |
| KDM6A D1216N-F | CCTGGAAATTTGGTCTGGATAAATGC |
| KDM6A D1216N-R | CCAAATTTCCAGGTCGCTGAATAAACCTA |
| KDM6A P1243dup-F | TTGGTCCACCACTTACAGCCTGCCAGTATAAATTG |
| KDM6A P1243dup-R | GGCTGTAAGTGGTGGACCAACATTCCAAGCAATGT |
| 3Myc-SND1-F | CTGTGGCAGAGGTAAAACTTC |
| 3Myc-SND1-R | GACAAGGTAACCCAGGTAGAAG |
| Flag-RPA1-F | TGACAAGCTCGAGATGGTCGGCCAACTGAGCGAG |
| Flag-RPA1-R | AACTAGTCTCGAGTCACATCAATGCACTTCTCCTG |
| KDM6A 1-400aa-F | GTTGTAGTTGAAAGGGCGAATTCGCGGCCGCACTCG |
| KDM6A 1-400aa-R | CCTTTCAACTACAACTTTTGCTTCTAGTTGCATTTAAG |
| KDM6A400-1000 aa-F | CTTCTGCAGGAATTCTGTAAACCTCATCATCCAAATAC |
| KDM6A400-1000 aa-R | CTGTCGACGATATCTTAGTTAGCTTCCACCAAAGTTTTAG |
| KDM6A1000-1401 aa-F | TACGCTCTCGAGCCCATGGATGCTTTACAGGCCTATA |
| KDM6A1000-1401 aa-R | ACTAGTCTCGAGTCAAGATGAGGCGGATGGTAATGGA |
| Myc-SND1 SN del-F | AGGGTGCTATGAGGAGCAGCCCGTGGAGGAGGTGAT |
| Myc-SND1 SN del-R | TCCTCATAGCACCCTGAGAGGACCATCTTGATGATGC |
| Myc-SND1 TSN del-F | CTCCTATTAACTCGAGACTAGTTCTAGAGCGGCC |
| Myc-SND1 TSN del-R | TCGAGTTAATAGGAGCCCTCTACAGGGGGGTGACT |
| GFP-SND1-F | AGGGTCCCTCGAGAATGGCGTCCTCCGCGCAGAG |
| GFP-SND1-R | GAATTAGTCTCGAGTTAGCGGCTGTAGCCAAATTCG |
| GFP-SND1 SN del-F | GGTCCCCTATGAGGAGCAGCCCGTGGAGGAGGT |
| GFP-SND1 SN del-R | TCCTCATAGGGGACCGCGGGTCCCCCGGAGGA |
| GFP-SND1 TSN del-F | GGGCTCCTATTAACTCGAGACTAATTCTAGAGC |
| GFP-SND1 TSN del-R | AGTTAATAGGAGCCCTCTACAGGGGGGTGACTG |
| GFP-RPA1-F | TTCCGGTGAATTCATGGTCGGCCAACTGAGCGAG |
| GFP-RPA1-R | ACACCATGAATTCCATCAATGCACTTCTCCTGATGCTC |