Table 1:

Model independent parameters from each nucleotide incorporation.

Parameter	AMP (Appling et al.) add to C	UMP Add to C	AMP Add to U	GMP Add to C	CMP Add to U
11-mer
${\mathsf{k}}_{\text{obs},\max }({\mathrm{s}}^{\text{-}1})$	270 ± 30	800 ± 200	290 ± 20	340 ± 50	340 ± 10
${\mathsf{K}}_{1∕2}(\mu \mathsf{M})$	170 ± 30	200 ± 100	60 ± 20	80 ± 40	69 ± 9
$<{\mathsf{k}}_{\text{obs},2}>({\mathrm{s}}^{\text{-}1})$	0.27 ± 0.02	1.7 ± 0.1	0.088 ± 0.001	0.4 ± 0.2	0.25 ± 0.02
Dimer
$<{\mathsf{k}}_{\text{obs}}>({\mathrm{s}}^{\text{-}1})$	0.40 ± 0.02	2.0 ± 0.1	0.094 ± 0.002	0.15 ± 0.02	0.26 ± 0.02