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. Author manuscript; available in PMC: 2025 Sep 1.
Published in final edited form as: Biophys Chem. 2024 Jun 8;312:107281. doi: 10.1016/j.bpc.2024.107281

Table 2:

Kinetic Parameters from Global Analysis using Scheme 1

Parameter AMP (Appling et
al.)
Add to C
UMP
Add to C
AMP
Add to U
GMP
Add to C
CMP
Add to U
knp,f(s-1) 0.075 ± 0.006 0.16 ± 0.06 20 ± 30 0.07 ± 0.01
knp,r(s-1) 0.02 ± 0.01 30 ± 50 0.002 ± 0.001
KD(μM) 96 ± 6 170 ± 20 80 ± 30 150 ± 30 106 ± 6
k3(s-1) 182 ± 4 630 ± 50 319 ± 8 400 ± 60 370 ± 10
k4(s-1) 9 ± 1 2 ± 1 3 ± 2 7 ± 2
k5(s-1) 0.35 ± 0.02 2.2 ± 0.1 0.092 ± 0.004 0.110 ± 0.002 0.301 ± 0.006
k6(s-1)-100 0.008 ± 0.006
k6(s-1)-300 0.13 ± 0.07 0.015 ± 0.005 0.031 ± 0.004
k6(s-1)-500 0.2 ± 0.1 0.026 ± 0.006 0.07 ± 0.02
k6(s-1)-1000 0.51 ± 0.07 0.046 ± 0.003 0.15 ± 0.02 0.03 ± 0.01
[EC10][EC10,total] 0.77 ± 0.03 0.89 ± 0.01 0.91 ± 0.02 0.82 ± 0.02