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. 2024 Jul 8;15:1403272. doi: 10.3389/fimmu.2024.1403272

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Copyright © 2024 Ni, Cui, Yang, Ding, Zhao, Hu, Zhan, Kang, Hu, Zhao, Xu, Chen, Liu, Zhao, Zhang, Huang, Li, Yang, Zhang, Zhang, Deng, Yang, Lu and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dynamic changes of G-MDSCs in patients after allo-HSCT within 90 days. (A) Gating strategy of granulocytic myeloid-derived suppressor cells (GMDSCs) and monocytic myeloid-derived suppressor cells (M-MDSCs). The frequencies of G-MDSCs in patients at day 14 (n=25), day 21 (n=20), day 28 (n=17), day 42 (n=16), day 60 (n=23), and day 90 (n=21) were analyzed using flow cytometry. (B) The absolute numbers of G-MDSCs were calculated as well. (C) The mean fluorescence intensity (MFI) and percentage of intracellular heme oxygenase-1 (HO-1) in G-MDSCs at day 28 were determined using intracellular staining. The absolute number of HO-1⁺ G-MDSCs at day 28 was calculated (n=17). Bars indicate the mean value of replicates, with error bars indicating the standard error of the mean.