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. 2024 Jul 8;15:1403272. doi: 10.3389/fimmu.2024.1403272

Figure 4.

Figure 4

The impact of G-MDSCs on autologous T cells and NK cells. (A) The secretion of interferon-gamma (IFN-γ) by CMV-specific T cells in the presence of granulocytic myeloid-derived suppressor cells (G-MDSCs) at day 14 (n=10), day 28 (n=7), day 60 (n=7), and day 90 (n=7) were measured by IFN-γ ELISpot assay. Bars indicate the mean value of replicates, with error bars indicating the standard error of the mean. (B, C) Proliferation of CD4-CD8+ and CD4+CD8- T cells in the presence of G-MDSCs at day 14 (n=11), day 28 (n=19), day 60 (n=12), and day 90 (n=12) were determined using flow cytometry after 4 days co-culture. Cell count and division index were determined, respectively. (D) G-MDSCs were co-cultured with T cells, in the presence of either the CMV pool or anti-CD3/28 for 8 hours. G-MDSCs incubated with T cells was used as control. The surface expression of HLA-DR was detected after incubation (n=11). Bars indicate mean value of replicates with error bars indicating standard error of the mean. (E) G-MDSCs from the patients at day 28 were co-cultured with T cells for 4 days, in the presence of anti-CD3/28. The proportion of Th subsets were evaluated using flow cytometry (n=9). *p=0.0377.