FIG. 7.
Localization of amino acid positions at which variations between NPs and RPs were observed on the tertiary structure of Nef. Residues H102, N157, and E182 were frequently found at these positions in NPs. The four α-helices (αa to αd), the four β-strands (βa to βd), and the approximate position of the (PxxP)3 motif are also indicated. Regions encompassing the remaining six amino acid positions used to define the NefProg score (residues 15, 39, 51, 63, 169, and 170) were deleted from the molecule used to determine the nuclear magnetic resonance solution structure of Nef (14).