Figure 3.

Phenotypic analysis of P. lilacinum grown on different C:N ratios (1:1, 2:1, 5:1, 10:1, 20:1, and 40:1). (A) Colony morphology and (B) colony size of 5-day-old cultures on minimal medium supplemented with different C:N ratios at 28°C. Exactly 1 μL of a 1 × 10⁶ conidia/mL suspension was spotted onto the center of the various media. Different lowercase letters indicate significant differences (p < 0.05). (C) SDS-PAGE analysis of extracellular proteins from P. lilacinum grown for 96 h under different C:N ratios. (D) Concentration of extracellular protein from P. lilacinum. (E) Zymography of gelatinase by secreted proteins of P. lilacinum grown under different C:N ratios during 96 h of liquid fermentation. (F) Caseinolytic activity of P. lilacinum grown under different C:N ratios. The values represent the mean of three biological replicates, with error bars indicating standard deviations.