Figure 1.

GATA6⁺ pericardial macrophages protect against eosinophilic pericarditis

(A) Schematic of experimental design.

(B) Representative histology of Lys^Cre and Lys^CreGATA6^fl/fl mice. Scale bar: 100 μm. Zoom: 10×.

(C) Histology showing isolated pericardium. Scale bar: 50 μm. Zoom: 20×.

(D) Nuclei present in each image of the pericardium (Lys^Cren = 5, Lys^CreGATA6^fl/fln = 4).

(E) Area of each pericardial histology section.

(F) Average thickness of the pericardium along the region of interest.

(G) Maximum thickness of the pericardium in the region of interest.

(H) Pie charts comparing major leukocyte compartments between Lys^Cre and Lys^CreGATA6^fl/fl mice (Lys^Cren = 10, Lys^CreGATA6^fl/fln = 10). Proportions are a percentage of CD45⁺ cells of whole heart with pericardium.

(I) Pie charts comparing Ly6C and CCR2 expression between Lys^Cre and Lys^CreGATA6^fl/fl mice. Proportions are a percentage of CD64⁺F4/80⁺ cells. ∗ indicates populations that are significantly different (p < 0.05).

(J) Ly6C⁺CCR2^- cells as a proportion of CD64⁺F4/80⁺ cells.

(K) Ly6C⁻CCR2⁺ cells as a proportion of CD64⁺F4/80⁺ cells.

(L) Schematic of isolation of the pericardial tissue.

(M) Pie charts comparing major leukocyte compartments between Lys^Cre and Lys^CreGATA6^fl/fl mice in the pericardial tissue alone. Proportions are a percentage of CD45⁺ cells. Statistics: Student’s t test with Welch’s correction. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.