Figure 2.

GATA6⁺ pericardial macrophages do not attenuate myocardial inflammation in myocardial infarction or coxsackievirus-B3 myocarditis

(A) Schematic of experimental design for MI.

(B) Pie charts comparing major leukocyte compartments between Lys^Cre and Lys^CreGATA6^fl/fl mice (Lys^Cren = 8, Lys^CreGATA6^fl/fln = 12) in the whole heart with pericardium and myocardium. Proportions are a percentage of CD45⁺ cells. ∗ indicates populations that are significantly different (p < 0.05).

(C) Ly6C⁺CCR2⁻ cells as a proportion of CD45⁺ cells.

(D) Ly6C⁻CCR2⁺ cells as a proportion of CD45⁺ cells.

(E) Pie charts comparing Ly6C and CCR2 expression between Lys^Cre and Lys^CreGATA6^fl/fl mice. Proportions are a percentage of CD64⁺F4/80⁺ cells. ∗ indicates populations that are significantly different (p < 0.05).

(F) Schematic of experimental design for CVB3 myocarditis.

(G) Myocarditis score of mice exposed to CVB3 (Lys^Cren = 9, Lys^CreGATA6^fl/fln = 10). Score 1: <10% inflammation, Score 2: 10–20% inflammation, Score 3: 30–50% inflammation, Score 4: 50–90% inflammation.

(H) Representative histology slides of Lys^Cre and Lys^CreGATA6^fl/fl mice. Scale bar: 200 μm. Zoom: 1.25×.

(I) Pie charts comparing major leukocyte compartments between Lys^Cre and Lys^CreGATA6^fl/fl mice. Proportions are a percentage of CD45⁺ cells. Statistics: Student’s t test with Welch’s correction. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.