FIG. 4.

Replication kinetics of chimeric viruses in PBMCs, macrophages, and T-cell lines. Virus stocks used to infect the indicated cell types were generated by transfecting HeLa cells with recombinant plasmid DNAs. The CD8-depleted PBMCs stimulated with PHA (A), macrophages (B), and T-cell lines MT2, H9, M8166, Molt4, and PM1 (C) were infected with each virus stock containing equal amounts of RT activity, and progeny virus production was monitored by RT activity released into the culture medium at the indicated time points. Each plot represents the average of duplicated or triplicated assays.