Fig. 1.

An integrated antigenic cassette identification workflow. The flow diagram shows the steps originating from extracting sequences from various databases, analyzing the sequences, and designing the codon-optimized sequence of the antigen. The critical attributes and the checkpoints at each step of the workflow like the assembly, cloning, plasmid, and mRNA preparation are shown. * denotes the 1.5 to 2 weeks of the oligonucleotide/gene synthesis, purification, and shipping from commercial suppliers.