Table 1.

qPCR reaction master mix for positive region

Reagent	Amount
2× SYBR Power up master mix	2.5 μL
Primer Fwd/Rev (5 pmol/μL) of actin gene	0.2 μL
Nuclease-free water	0.8 μL
Template (Immunoprecipitated DNA)	1.5 μL