Reagents	Final concentration	Amount
RIPA zero-SDS buffer	N/A	930 μL
SDS (10%)∗	0.1%	10 μL
PIC (50×)∗	1×	20 μL
PMSF (50 mM)∗	1 mM	20 μL
NaBu (1M)∗	20 mM	20 μL
Total	N/A	1000 μL

∗ Add just before use