Reagents	Final concentration	Amount
Tris-HCl (pH 8.1) (0.5 M)	20 mM	40 μL
NaCl (5.0 M)	500 mM	100 μL
EDTA (0.1 M)	2 mM	20 μL
Triton X-100 (100%)	1% (vol/vol)	10 μL
SDS (10%)∗	0.1%	10 μL
PIC (50×)∗	1×	20 μL
PMSF (50 mM)∗	1 mM	20 μL
NaBu (1M)∗	20 mM	20 μL
Nuclease-free water	N/A	760 μL
Total	N/A	1000 μL

∗ Add just before use