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| Reagents | Final concentration | Amount |
|---|---|---|
| Tris-HCl (pH 8.1) (0.5 M) | 10 mM | 20 μL |
| Deoxycholate (100%) | 1% | 10 μL |
| EDTA (0.1 M) | 1 mM | 10 μL |
| NP-40 (100%) | 1% (vol/vol) | 10 μL |
| LiCl (1 M) | 0.25 M | 25 μL |
| SDS (10%)∗ | 0.1% | 10 μL |
| PIC (50×)∗ | 1× | 20 μL |
| PMSF (50 mM)∗ | 1 mM | 20 μL |
| NaBu (1M)∗ | 20 mM | 20 μL |
| Nuclease-free water | N/A | 855 μL |
| Total | N/A | 1000 μL |
∗ Add just before use
