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. 1999 Jul;73(7):5556–5567. doi: 10.1128/jvi.73.7.5556-5567.1999

FIG. 4.

FIG. 4

FIG. 4

FIG. 4

FIG. 4

FIG. 4

Kinetics of the expression of KSHV IE mRNAs following induction of viral reactivation in BC-1 and BCBL-1 cells. Cells were treated with sodium butyrate for 0, 4, 6, 8, 12, 24, and 48 h. Total RNA was isolated at each time point and analyzed by Northern blotting. RNA blots were hybridized with single-stranded DNA complementary to ORF50 mRNAs (A), single-stranded DNA complementary to ORF45 mRNA (B), single-stranded DNA complementary to ORF K4.2 mRNA (C), single-stranded DNA complementary to nucleotides 50016 to 50261 (the 4.5-kb mRNA) (D), and random priming-labeled KSHV ORF59 cDNA (E). In each hybridization, random priming-labeled cDNA of RNase P RNA was included to serve as a control for loading. Molecular markers were an 0.24- to 9.5-kb RNA ladder (sizes are given in kilobases to the left of each panel).