FIG. 6.

RT-PCR of ORF50-K8-K8.2 mRNA. Poly(A)⁺ RNAs were extracted from sodium butyrate-induced BC-1 cells and used to perform RT-PCR with two primers adjacent to introns 2 and 3 of ORF50-K8-K8.2 mRNA (lane 2). A PCR without reverse transcription was also performed with the same RNA and primers as a control (lane 1). The size of three RT-PCR fragments in lane 2 is in agreement with the size of fragments amplified with cloned cDNAs for type I (lane 3), type II (lane 4), and type III (lane 5) ORF50-K8-K8.2 mRNAs. Lane M is the molecular markers, a 100-bp DNA ladder (Promega).