| Step | Reagent | Time (min:sec) |
|---|---|---|
| 1 | 70% ethanol | 30:00 |
| 2 | 80% ethanol | 30:00 |
| 3 | 95% ethanol | 30:00 |
| 4 | 100% ethanol | 30:00 |
| 5 | 100% ethanol | 60:00 |
| 6 | 100% ethanol | 90:00 |
| 7 | Xylene | 45:00 |
| 8 | Xylene | 45:00 |
| 9 | Xylene | 90:00 |
| 10 | Paraffin | 60:00 |
| 11 | Paraffin | 60:00 |
| 12 | Paraffin | 90:00 |
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