Fig. 5.

TAF1 protein expression in the mouse brain. (A-C) Comparison of TAF1 protein expression in non-recombinant mice, recombinant 1loxP heterozygous female mice and Wt littermates at 6-7 months of age. The presence of non-recombinant loxP sites did not alter TAF1 protein expression (A) and TAF1 abundance was not altered in 1loxP heterozygous female mice compared to that in littermates (2loxP heterozygous, 2loxP homozygous, 1loxP heterozygous and Wt mice; n=6/genotype) (B) or in 2loxP hemizygous male mice compared to that in Wt littermates (2loxP hemi, Wt; n=4/genotype) (C). Error bars show mean±s.e.m. Statistical analysis using one-way ANOVA with Tukey's multiple comparison test was performed for B and unpaired two-tailed t-test for C; P-values are indicated. The experiment was replicated three times or more during the optimisation stage.