FIG. 3.

Growth of ICP22 mutants in Vero and HEL cells. Confluent monolayers of Vero (A) or HEL (B) cells in 25-cm² flasks were infected in duplicate with WT HSV-1 strain (KOS1.1) or various HSV-1 mutants at an MOI of 10 and incubated at 37°C for 24 h. Virus yield in the infected-cell lysates was determined by plaque assay on Vero cells. Each bar denotes the mean virus yield for the cells infected with the indicated virus, with the error bars indicating the values of the two duplicate infections.