FIG. 5.

Schematic representation of WT and mutant UL13 alleles. (A) Representation of the prototypical form of the HSV-1 genome. Unique DNA sequences are represented by horizontal lines, and inverted-repeat DNA sequences are shown as open and gray bars. The position of the BglII O fragment, containing the UL13 gene, is indicated, and this fragment is enlarged in panel B. (B) Map of the WT BglII O fragment. HSV-1 DNA sequences are denoted by the horizontal line. Above, the UL13 and UL14 transcripts are denoted by arrows, and the UL13 and UL14 ORFs are indicated by open bars. Below, the DNA sequences which are deleted in d13-lacZ are shown as a black bar. (C) Map of the altered BglII O fragment present in d13-lacZ. DNA sequences are shown at the bottom. HSV-1 sequences are represented by a horizontal line, and E. coli lacZ sequences are denoted by a crosshatched bar. Above, transcripts and ORFs of UL14 and the UL13–β-galactosidase fusion protein (beta-gal) are indicated as in panel B. Restriction sites relevant to the engineering and analysis of d13-lacZ are indicated in panels A and B: A, AgeI; Bg, BglII; Bs, BstEII; E, EcoRI; Ea, EagI; H, HindIII; Nh, NheI).