FIG. 7.

Immunoblot analysis of RNAP II large-subunit forms in Vero cells infected with d13-lacZ. Vero cells were mock infected (lanes 1) or were infected with the virus strains indicated for 4 or 8 h. Cells were scraped, washed, and lysed directly into SDS-PAGE sample buffer. Protein extracts were run on SDS–6% PAGE, transferred to filters, and probed with ARNA3 (A) or 8WG16 (B). All lanes in panel A or B contain extracts from the same numbers of cells. The positions at which various RNAP II large-subunit forms migrate are indicated.