TABLE 3.
Vγ/Vδ usage in transgenic micea
| Strain | No. of mice/group | Tissue | Mean ± SEM
|
|||||
|---|---|---|---|---|---|---|---|---|
| Total lymphoid cells (105) | Total γδ+ (GL3-3A+) cells (105) | Positive cells/animal (105)
|
||||||
| Vγ1+ | Vγ4+ | Vδ4+ | Vδ6.3+ | |||||
| C57BL/6 | 3 | Heart | 8.7 ± 0.04 | 2.8 ± 0.9 (32b) | 1.6 ± 0.03 (18) | 0.2 ± 0.1 (2) | 0.5 ± 0.3 (6) | 0.6 ± 0.2 (7) |
| 4 | Spleen | 715.9 ± 63.5 | 45.2 ± 21.0 (6) | 9.4 ± 3.5c (1) | 2.1 ± 0.09 (0.3) | 4.0 ± 1.6 (0.1) | 4.8 ± 2.0 (0.1) | |
| Bl.Tg.Eα | 4 | Heart | 47.4 ± 1.8 | 18.2 ± 3.9 (39) | 2.3 ± 0.4 (5) | 5.2 ± 1.8c (11) | 3.3 ± 0.8 (7) | 1.4 ± 0.05 (3) |
| 6 | Spleen | 405.06 ± 23.5 | 61.5 ± 16.6 (15) | 4.7 ± 2.0 (1) | 10.3 ± 2.7c (3) | 3.2 ± 1.6 (1) | 5.7 ± 2.3 (1) | |
a
Lymphoid cells were isolated from spleens and hearts of individual mice 7 days after infection with CVB3 and depleted of RBC as indicated in Materials and Methods. Aliquots of cells were stained for a total γδ+ cells or Vγ/Vδ subpopulations.
b
Percentage of total lymphoid cells positive for the marker.
c
Total positive cells recovered per tissue differs from same tissue of other strain at P ≤ 0.05 by Wilcoxon ranked score.