TABLE 4.
Th1/Th2 phenotype of CD4+ T cells in transgenic micea
| Strain | No. of mice/group | Mean ± SEM
|
|||||
|---|---|---|---|---|---|---|---|
| Total splenocytes (106) | Positive cells/spleen (106)
|
||||||
| CD4+ | IFN-γ+ | IL-4+ | CD4+ IFN-γ+ | CD4+ IL-4+ | |||
| C57BL/6 | 6 | 86.5 ± 13.7 | 11.9 ± 2.5 | 0.7 ± 0.4 (0.39b) | 1.8 ± 0.4 | 0.1 ± 0.1 (0.13) | 0.8 ± 0.3 |
| Bl.Tg.Eα | |||||||
| Untreated | 4 | 50.1 ± 12.4 | 6.0 ± 1.3 | 2.3 ± 0.9c (3.29) | 0.7 ± 0.3c | 0.5 ± 0.2c (2.5) | 0.2 ± 0.1c |
| Treated with anti-γδ TcR | 4 | 68.1 ± 10.7 | 8.9 ± 2.2 | 1.6 ± 0.4 (0.84) | 1.9 ± 0.5 | 0.3 ± 0.2 (0.60) | 0.5 ± 0.2 |
a
Bl.Tg.Eα mice were treated with a total of 200 μg of anti-γδ TcR antibody or hamster IgG as an isotype control. Spleen cells from individual mice obtained 7 days after infection with CVB3 were stained for CD4 and cytokines as described in Materials and Methods.
b
Ratio of IFN-γ+ cells to IL-4+ cells.
c
Number of positive cells significantly different from C57BL/6 at P ≤ 0.05 by Wilcoxon ranked score.