Table 2.
The enzymatic properties of GAGases
| Condition | GAGase I | GAGase II | GAGase III | GAGase IV | GAGase V | GAGase VI | GAGase VII | GAGase VIII | |
|---|---|---|---|---|---|---|---|---|---|
| Optimal temperature (°C) | 40 | 30 | 40 | 40 | 40 | 50 | 40 | 40 | |
| Optimal buffer (pH) | Tris–HCl (pH 7.0) | Tris–HCl (pH 7.0) | Tris–HCl (pH 7.0) | Tris–HCl (pH 7.0) | NaAc-HAc (pH 6.0) | Tris–HCl (pH 8.0) | NaAc-HAc (pH 6.0) | Tris–HCl (pH 8.0) | |
| Effect of alkaline metal ions (5 mM) | KCl (104%) | KCl (104%) | KCl (120%) | KCl (139%) | NaCl (108%) | NaCl (113%) | ND | NaCl (107%) | |
| NaCl (109%) | NaCl (118%) | ||||||||
| Effect of metal cation (5 mM) | Enhancer | Mn2+ (107%) | Ca2+ (137%) | Ca2+ (173%) | Mg2+ (104%) | ND | ND | Ca2+ (102%) | Mn2+ (105%) |
| Ba2+ (105%) | Mg2+ (145%) | Mg2+ (114%) | Mn2+ (126%) | Mn2+ (103%) | Ba2+ (108%) | ||||
| Mn2+ (116%) | Mn2+ (212%) | ||||||||
| Inhibitor | Cr3+ (0%) | Pb2+ (0%) | Hg2+ (0%) | Fe3+ (0%) | Hg2+ (0%) Cu2+ (0%) | Cr3+ (0%) | Fe3+ (0%) | Fe3+ (0%) | |
| Pb2+ (0%) | Cu2+ (0%) | Fe3+ (0%) | Cr3+ (0%) | Zn2+ (0%) | Zn2+ (0%) | Cr3+ (0%) | Cr3+ (0%) | ||
| Fe2+ (0%) | Zn2+ (0%) | Cu2+ (0%) | Pb2+ (0%) | Cr3+ (8%) | Pb2+ (0%) | Pb2+ (0%) | |||
| Zn2+ (0%) | Cr3+ (4%) | Zn2+ (0%) | Cu2+ (4%) | Cu2+ (23%) | Cu2+ (0%) | ||||
| Cu2+ (6%) | Fe3+ (5%) | Pb2+ (3%) | Fe2+ (8%) | Zn2+ (28%) | Zn2+ (7%) | ||||
| Hg2+ (10%) | Hg2+ (9%) | Ni2+ (13%) | Zn2+ (12%) | Ni2+ (10%) | |||||
| Effect of (5 mM) EDTA | 60% | 24% | 78% | 65% | 88% | 84% | 73% | 73% | |
| Specific enzyme activity (U/mg) | HA | 5.62 ± 0.22 | 2.81 ± 0.23 | 16.47 ± 1.03 | 13.63 ± 2.24 | 0.39 ± 0.04 | 0.10 ± 0.01 | ND | ND |
| CS-C | 0.83 ± 0.25 | 0.62 ± 0.07 | 3.96 ± 0.22 | 5.61 ± 0.29 | 0.25 ± 0.01 | 0.13 ± 0.01 | ND | ND | |
| HS | 0.08 ± 0.05 | 0.28 ± 0.03 | 0.33 ± 0.09 | 0.71 ± 0.25 | 0.06 ± 0.04 | ND | ND | ND | |
| Alginate | ND | ND | 0.29 ± 0.07 | ND | ND | ND | ND | ND | |
Abbreviation: ND, not detected.