Figure 2. Immature feeding strategies limit LTM.

(A) Feeding rate of L3 expressing npf-Gal4 >UAS-TrpA1 and genetic controls at 30 °C at CT13. (B–D) Sleep duration (B), bout number (C), and bout length (D) of L3 expressing npf-Gal4 >UAS-TrpA1 and genetic controls at 30 °C. (E–G) Arousal threshold (E), long-term aversive memory performance (F), and short-term aversive memory performance (G) in L3 expressing npf-Gal4 >UAS-TrpA1 and genetic controls at 30 °C. (H,I) Short-and long-term term aversive memory performance in L3 raised on ctrl and L.S. food. A, n=18–20 larvae; B-D, n=24–61 larvae; E, n=125–160 sleep episodes, 18 larvae per genotype; F-I, n=8 PIs (240 larvae) per genotype. One-way ANOVAs followed by Sidak’s multiple comparisons tests (A) and (E–G) Two-way ANOVAs followed by Sidak’s multiple comparison test (B–D) unpaired two-tailed Student’s t-test (H and I). Source data in Figure 2—source data 1.

Figure 2—figure supplement 1. Baseline odor preferences, feeding, and sleep are not affected by npf-Gal4 manipulations.

(A) Feeding rate of L3 expressing npf-Gal4 >UAS-TrpA1 and genetic controls at 22 °C at CT13. (B–E) Sleep duration (B), bout number (C), bout length (D), and arousal threshold (E) in L3 expressing npf-Gal4 >UAS-TrpA1 and genetic controls at CT1 and CT13 at 22 °C (temperature controls). (F) Long-term aversive memory performance in L3 expressing npf-Gal4 >UAS-TrpA1 and genetic controls at 22 °C (temperature controls). (G–I) Naïve OCT, AM, and quinine preference in L3 expressing npf-Gal4 >UAS-TrpA1 and genetic controls at 30 °C. (A) n=18–20 larvae; (B-D) n=22–27 larvae; (E) n=120–205 sleep episodes, 18 larvae per genotype; (F) n=8 PIs (240 larvae) per genotype; (G-I) n=6 PREFs (180 larvae) per genotype. One-way ANOVAs followed by Sidak’s multiple comparisons tests (A), (E), and (F–I) Two-way ANOVAs followed by Sidak’s multiple comparison test (B–D). Source data in Figure 2—figure supplement 1—source data 1.