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. 2024 Jul 22;13:RP97256. doi: 10.7554/eLife.97256

Figure 3. Deeper sleep in L2 is energetically disadvantageous.

(A, B) Sleep duration (A) and arousal threshold (B) of L2 control fed vehicle control (L2) or Gaboxadol (L2 Gab). (C, D) Total body weight of L2 (C) (in groups of 10) or L3 (D) (in groups of 5) fed vehicle control or Gaboxadol (Gab). (E) Long-term aversive memory performance in L2 fed vehicle control (L2) or Gaboxadol (L2 Gab). (F, G) Sleep duration (F) and arousal threshold (G) of L2 expressing R76G11-Gal4>UAS-TrpA1 and genetic controls at 30 °C. (H) Long-term aversive memory performance of L2 expressing R76G11-Gal4>UAS-TrpA1 and genetic controls at 30 °C. (I, J) Total body weight (I) and total body length (J) of L2 expressing R76G11-Gal4>UAS-TrpA1 and genetic controls at 30 °C. (A) n=28 larvae; (B) n=110–220 sleep episodes, 18 larvae per genotype; (C) n=5–7 groups (50–70 larvae); (D) n=8 groups (40 larvae); (E) n=8 PIs (240 larvae) per genotype; (F) n=33–36 larvae; (G) n=234–404 sleep episodes, 30–40 larvae per genotype; (H) n=8 PIs (240 larvae) per genotype; (I) n=5 groups (25 larvae); (J) n=31–32 larvae. Unpaired two-tailed Student’s t-tests (A–E) one-way ANOVAs followed by Sidak’s multiple comparisons tests (F–J). Source data in Figure 3—source data 1.

Figure 3—source data 1. Sleep, LTM, and developmental data for L2 manipulations.

Figure 3.

Figure 3—figure supplement 1. Baseline sleep and odor preferences are not disrupted by R76G11-Gal4 manipulations.

Figure 3—figure supplement 1.

(A, B) Sleep bout number (A) and bout length (B) of L2 control fed vehicle control (L2) or Gaboxadol (L2 Gab). (C, D) Sleep bout number (C) and bout length (D) of L2 expressing R76G11-Gal4>UAS-TrpA1 and genetic controls at 30 °C. (E–H) Sleep duration (E), bout number (F), bout length (G), and arousal threshold (H) of L2 expressing R76G11-Gal4>UAS-TrpA1 and genetic controls at 22 °C (temperature controls). (I–K) Naïve OCT, AM, and quinine preference in L2 expressing R76G11-Gal4>UAS-TrpA1 and genetic controls at 30 °C. (L) Short-term aversive memory performance of L2 expressing R76G11-Gal4>UAS-TrpA1 and genetic controls at 30 °C. (A, B) n=28 larvae; (C-D) n=33–36 larvae; (E-G) n=27–29 larvae; (H) n=145–316 sleep episodes, 30–40 larvae per genotype; (I-K) n=6 PREFs (180 larvae) per genotype; (L) n=8 PIs (240 larvae) per genotype. Unpaired two-tailed Student’s t-tests (A–B) One-way ANOVAs followed by Sidak’s multiple comparisons tests (C–L). Source data in Figure 3—figure supplement 1—source data 1.
Figure 3—figure supplement 1—source data 1. Temperature control and naive preference data for L2 manipulations.