Figure 4.

HJXJ alleviates PS-induced ROS production, pyroptosis, and NLRP3 inflammasome activation in MPC5 cells. MPC5 cells are treated with 1 mM PS in the absence or presence of different HJXJ concentrations. Flow cytometry was used to evaluate (a) ROS production and (b, c) pyroptosis. Western blotting is used to measure expressions of NLRP3, Caspase-1 p20, GSDMD-N, podocin, and nephrin (d, e). ELISA kits are used to measure (f) release of IL-1β and IL-18. ^∗∗∗P < 0.001 versus control. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 versus PS.