Figure 5.

HJXJ alleviates Nlrp3 overexpression-induced pyroptosis and NLRP3 inflammasome activation in MPC5 cells. MPC5 cells are transduced with Nlrp3 expression vector or blank vector. (a) The NLRP3 mRNA expression is measured using quantitative real-time PCR, and (b) protein expression is measured using western blotting. MPC5 cells are pretreated with 10% HJXJ and transduced with Nlrp3 expression vector. Flow cytometry is used to evaluate (c, d) pyroptosis level. Western blotting is used to measure (e, f) expressions of NLRP3, Caspase-1 p20, and GSDMD-N. ELISA kits are used to measure (g) release of IL-1β and IL-18^.∗∗∗P < 0.001 versus vector. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 versus oeNlrp3.