FIG. 3.

RIPA analysis of Env glycoproteins expressed upon transfection of 293T cells with env expression vectors containing mutated or wild-type env genes. Cells were ³⁵S labelled overnight, and Env glycoproteins were immunoprecipitated with a mixture of anti-gp120 and anti-gp41 sheep antibodies. The names of the Env mutants are indicated above the lanes. M, mock transfection. Molecular mass standards are indicated on the left. (A) HIV-1 ADA Env mutants. The weak gp41 band detected for wild-type ADA Env and the p34A mutant was also visible for the t7A, t24A, and t25A mutants after overexposure of the film (data not shown). (B) HIV-1 LAI Env mutants.