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RIPA analysis of wild-type or mutant HIV-1 ADA Env glycoproteins expressed upon transfection of HeLa P5 cells. M, mock transfection. RIPA was performed on cell lysates (A) or supernatants (B). Note the presence of a band unrelated to the HIV-1 Env glycoproteins in the supernatants of all transfections, including the mock transfection.
