FIG. 6.

ELISA analysis of Env glycoproteins in viral pellets of HIV-1 LAI wild-type (W) or mutant viruses from supernatants of transfected 293T cells. Either anti-gp120 D7324 (A) or anti-gp41 D7323 (B) was used as the capture antibody. Tests were run in duplicate; the mean values are shown. p24 in viral pellets was measured with a commercial ELISA kit. Three independent experiments were performed and yielded similar results; a representative assay is shown. (A) The amount of gp120 in viral pellets was quantified with recombinant gp120 as a calibration standard. Results are expressed as nanograms of gp120 per microgram of p24. (B) Anti-gp41 reactivity was measured in a sample dilution (0.7 μg of p24 per ml) which produced optical density (OD) values in the dynamic range of the curve.