TABLE 3.

Syncytium formation induced by LAI Env mutants^a

Clone	No. of syncytia	No. of nuclei/syncytium (range)
Wild type	20,648 ± 1,227	2–>30
FivL	4,949 ± 937	2–5
SivL	11,677 ± 3,490	2–5
t35L	8,429 ± 1,071	2–5
p34L	18,405 ± 2,555	2–>30
t7L	20,648 ± 2,237	2–>30
t24L	22,349 ± 2,806	2–>30
t25L	18,560 ± 1,448	2–>30

^a293T cells plated in six-well plates were transfected in triplicate with LAI wild-type Env or Env mutants. After coculturing with HeLa P5 cells (CD4⁺ CCR5⁺), fusion events were detected by β-galactosidase staining. Syncytia were counted independently by two investigators using a grid (486 squares/well). Mean ± standard deviation numbers of syncytia in three wells are shown. In three wells with mock-transfected cells, there were fewer than 10 syncytia.