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. 2024 Jul 9;15:1399718. doi: 10.3389/fpls.2024.1399718

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Copyright © 2024 Arya, Narayana, Sinha, Kandan and Satyanarayana Raju

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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2% TAE agarose gel images displaying a replication of the cross-amplification test for the most effective SSP (DorFP1/DorRP1) at a range of annealing temperatures (°C) [(A): 60, (B): 61, (C): 62, (D): 63, (E): 64, (F): 65, (G): 66, (H): 67, (I): 68 and (J): 69] by representing a single clear band of 506 bps in each lane. L: 100 bps molecular ladder (BR Biochem), lane 1: B. dorsalis, lane 2: B. zonata, lane 3: B. correcta, lane 4: Z. cucurbitae, lane 5: Z. tau and lane 6: B. digressa. It represents the SSP showing no cross-amplification with the DNA templates of the tested fruit fly species at 66°C annealing temperature.