Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jul 9;15:1399718. doi: 10.3389/fpls.2024.1399718

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2024 Arya, Narayana, Sinha, Kandan and Satyanarayana Raju

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

An optimized qPCR assay performed to assess the specificity of the DorFP1/DorRP1 SSPs using laboratory-extracted DNA templates from populations of B. dorsalis from UP (both maggot and adult), UK, HP, AS, WB, and BR. This was compared with B. correcta, B. zonata, B. digressa, Z. cucurbitae, and Z. tau, with inclusion of two NTC. (A) The graph illustrates the change in fluorescence signal (ΔRN) against the number of PCR cycles, representing successful amplification of samples containing B. dorsalis template DNA. (B) Melting curve analysis demonstrating species specificity of SSP for B. dorsalis, plotted against fluorescence derivative units (-RN’), and temperature (°C).