FIG. 4.

Test for spontaneous angularization of ts1201 capsids. Thirty-five-millimeter-diameter plates of BHK C13 cells were infected with 5 PFU of ts1201 per cell for 10 h at 39°C. They were then overlaid with fresh, prewarmed medium containing 200 μg of cycloheximide per ml and incubated for a further 14 h at 39°C as described for Fig. 3. The cells were then either harvested for electron microscopy (a) or incubated in the presence of cycloheximide for a further 4 h at 31°C (b) or 0°C (c). fc, free cores. Scale bar = 500 nm.