FIG. 1.

The K12 region is transcribed in BCBL-1 cells as a 2.3-kb mRNA. (A) Diagram of the ORF K12 region of BCBL-1 cells, including DRs DR1 and DR2, shown with relevant restriction sites and genomic coordinates derived from the sequenced BC1 isolate (41). The locations of the T0.7 mRNA (51) and BCBL-1 cDNA sequences are shown above the line; the locations of probes used for RNA mapping by Northern blotting are shown below. Note that the transcripts are depicted in a rightward orientation but are leftward relative to the numbered genome of the sequenced BC1 isolate. (B) Northern blotting of uninduced (−) and TPA-induced (+) BCBL-1 (Bcbl) and BJAB (BJ; KSHV negative) RNA with a T0.7 antisense riboprobe which detects the 2.3-kb mRNA species. (C) RNA mapping of the 2.3-kb mRNA in BCBL-1 cells with probes P1, P5, and P6. Abbreviations are the same as those for panel B.