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. 2024 Jul 22;15(7):522. doi: 10.1038/s41419-024-06902-4

Fig. 3. Erianin induces ferroptosis in TMZ-resistant GSCs.

Fig. 3

a, b Analysis of cell death and apoptosis in U87R and GSCm01R after erianin treatment, using annexin V FITC/PI staining. c, d Inhibitory measured using CCK-8 in U87R and GSCm01R cells 24 h after treatment with erianin and with or without Z-VAD-FMK, CQ, Nec-1, Fer-1. e Representative images of U87R cell morphological changes and GSCm01R neurosphere formation size with erastin treatment, erianin treatment, and subsequent Fer-1 and GSH treatment. Scale bar = 100 μm. f, g Measurement of ROS levels in U87R and GSCm01R after erianin treatment, using flow cytometry. h Measurement of intracellular GSH levels in U87R and GSCm01R treated with erianin. i Measurement of intracellular MDA levels in U87R and GSCm01R treated with Erianin. j, k ferroptosis detection in U87R and GSCm01R treated with Erianin, using BODIPY (581/591) C11 probe, and quantification of relative fluorescence intensity by Image J (k). Scale bar = 100 μm. l TEM used to observe ferroptosis in U87R and GSCm01R treated with Erianin (original magnification: ×100). m Expression of key ferroptosis regulators detected by western blotting in U87R and GSCm01R treated with Erianin. All data are shown as the mean ± SD (five independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001; ns no significance.