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. 2024 Jul 22;15(7):522. doi: 10.1038/s41419-024-06902-4

Fig. 6. Erianin promotes TMZ sensitivity in TMZ-resistant GSCs through REST.

Fig. 6

a–d Western blotting and qPCR assays showing REST protein (a, b) and mRNA expression (c, d) in normal, TMZ resistant, and REST overexpressed U87R and GSCm01R. e, f Cell viability measured with CCK-8 in REST overexpressed U87R and GSCm01R after combined treatment with TMZ and erianin. g, h Representative EdU assay showing proliferation and quantitative analysis in REST overexpressed U87R and GSCm01R after combined treatment with erianin and TMZ. Scale bar = 50 μm. i, k Migration assays of REST overexpressed U87R and GSCm01R using a Transwell 24-well plate without extracellular matrix gel after combined treatment. Scale bar = 50 μm. j, l Invasion assays of REST overexpressed U87R and GSCm01R using a Transwell 24-well plate with extracellular matrix gel after combined treatment. Scale bar = 50 μm. m, n ferroptosis detection by BODIPY (581/591) C11 probe in U87R and GSCm01R with combined treatment after REST overexpression and quantification of relative fluorescence intensity by Image J. Scale bar = 100 μm. o, p NSFA revealing the size of neurospheres and quantitative analysis of REST overexpressed GSCm01 after combined treatment with TMZ and erianin. Scale bar = 50 μm. q ELDA showing the neurosphere-forming ability of REST overexpressed GSCm01R after combined treatment with TMZ and erianin. r Western blotting of stemness marker in REST overexpressed GSCm01R after combined treatment with TMZ and erianin. All data are shown as the mean ± SD (five independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001; ns no significance.