FIG. 5.

Effect of low-pH citrate washing on uninfected L929 cells. L929 cells were treated with a citrate wash, new medium was added, and the cells were stained for FACS analysis immediately (A and D) or incubated at 37°C for 6 h in the presence (B and E) or absence (C and F) of cycloheximide (CHX) (500 μg/ml) (C and F) and stained for D^k (A to C) or K^k (D to F; with monoclonal antibody 11-4.1). Lines: light, unwashed cells; dark, washed cells.