Figure 3. HIV-1 infection induces translocation of cleavage and polyadenylation specificity factor subunit 6 (CPSF6) and CPSF5 to nuclear speckles.
The cellular changes observed in CPSF6, CPSF5, and lens epithelium–derived growth factor (LEDGF)/p75 localization following HIV-1 infection are illustrated. In uninfected cells, CPSF6, CPSF5, and LEDGF/p75 are distributed throughout the nucleus (left). Upon HIV-1 infection (right), CPSF6 and CPSF5 are translocated into nuclear speckles, subnuclear structures without membranes that behave like biomolecular condensates. LEDGF/p75 appears to surround nuclear speckles following HIV-1 infection (right).