FIG. 3.

HBV RNA-binding proteins are recognized by anti-La-positive human serum. Five micrograms of liver nuclear extract prepared from untreated or CTL-injected mice (lanes 1 and 2) and 5 μg of liver nuclear extract from untreated or CTL-injected mice after immunoprecipitation with control human serum (lanes 3 and 4) or with anti-La-positive human serum (lanes 5 and 6) were incubated with 40 fmol of in vitro-labeled RNA.B. The UV cross-linking reaction was performed as described in Materials and Methods. The gel was transferred to a nitrocellulose membrane and analyzed for La protein by Western blotting (WB) and by autoradiography to detect p45, p39, and p26 as described in Materials and Methods.