Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jul 5;27:101147. doi: 10.1016/j.mtbio.2024.101147

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2024 The Authors. Published by Elsevier Ltd.

This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

PMC Copyright notice

Fig. 7 — Polarization mechanism of the piezoelectric effect.

(A) Heatmap visualization of RNA-seq results and cluster analysis of differentially expressed genes (DEGs). Red indicates upregulated genes; blue indicates downregulated genes. (B) Volcano plots of the gene expression profile in macrophages: Ctr versus BMC and BMC versus BMC + L groups; up, ns, and down indicate upregulated genes, nonsignificant genes, and downregulated genes, respectively. (C) Gene ontology analysis of all genes in macrophages cultured on BMC membranes with LIPUS stimulation versus without LIPUS stimulation. (D) Enriched KEGG pathways of BMC versus BMC + L. (E) Heatmap of typical DEGs associated with macrophage polarization. (F) Immunofluorescence images and quantification analysis of Piezo1 expression in macrophages. Scale bar = 50 μm. (G) Detection of intracellular calcium content by flow cytometry. (H) Immunofluorescence analysis of CD163 and iNOS expression in macrophages under various treatments. Scale bar = 30 μm. (I) Schematic summary of the macrophage polarization pathways induced by BMC + L. L, LIPUS stimulation; **P < 0.01, ****P < 0.0001.