Figure 1.

Maternal HFD impacts glucose and cholesterol metabolism in offspring

(A) Experimental setup assessing the effects of maternal HFD before and throughout pregnancy on metabolic and circadian changes in offspring.

(B) Offspring birthweight.

(C) Percentage change in offspring body weight throughout the study.

(D and E) Glucose tolerance test (left) and the corresponding area under the curve (right, each color represents different litter) for 4-week-old offspring (D) and 16-week-old offspring (E). N = 5–6 mice (from different litters) per experimental group. All boxplots are Tukey boxplots and data is assessed with a Student’s t-test; line chart data are presented as mean ± S.E.M. and are analyzed via a repeated measure two-way ANOVA or mixed linear model followed by Šídák post hoc tests. The details of the statistical analysis results are available in Table S6. Ctr: maternal control diet (black); HFD: maternal high-fat diet (red).