Figure 6.
Differential gene expression in offspring exposed to maternal HFD
(A and B) GO enrichment analysis of hepatic genes showing a mean increase in expression in 4-week-old (A) and 16-week-old offspring upon maternal HFD (B).
(C) Volcano plots illustrating sex-biased differentially expressed genes in the livers of 16-week-old offspring upon maternal HFD.
(D) Barcode plots for male-biased genes in the liver of 16-week-old offspring with genes ordered from most down to most upregulated.
(E) Experimental design of Zheng et al.33
(F) Changes in offspring bodyweight from 4 to 32 weeks of age.
(G) Area under the curve for the glucose tolerance test and blood glucose levels at 30-, 60-, and 120-min post-glucose load (2 g/kg) in 32-week-old offspring. N = 2–3 mice (from different litters) per group. All boxplots are Tukey boxplots and line chart data are presented as mean ± S.E.M. Data are analyzed via two-way ANOVA followed by Holm-Šídák post hoc tests. The details of the results of the statistical analyses are available in Table S6. ∗∗p < 0.05 and ∗∗∗∗p < 0.0001, mHF-oHF vs. mHF-oCtr; ##p < 0.01 and ####p < 0.0001, mCtr-oHF vs. mCtr-oCtr; & p < 0.05, mCtr-oHF vs. mHF-oHF. mCtr, maternal control diet; mHFD, maternal high-fat diet; oCtr, offspring control diet; oHFD, offspring high-fat diet.