FIG. 4.

(A) MAb 17b/HXB2-gp120 competition assay by SPR. Sequential injections of HXB2-gp120 (at 200 nM) with peptide at increasing concentrations (0.2 to 200 μM) were made over a surface with 1,500 RU of coupled MAb 17b. The results are given as percentage of gp120 bound relative to gp120 bound in the absence of peptide. (B) gp120 bound to 1,500 RU of coupled MAb 17b when 200 nM solutions of gp120 were injected after mixing with 12p1 (210 μM), 12p1[Scrambled] (200 μM), 12p3 (202 μM), 12p2 (240 μM), 12p2[Scrambled] (272 μM), 12p2[P3/A] (220 μM), 12p2[Y4/A] (277 μM), 12p5 (50 μM), 12p6 (50 μM), C7Cp1 (176 μM), 7p1 (204 μM), 7p2 (196 μM), and 12p1[2–8] (301 μM).