TABLE 2.
IC50s for inhibition of the 4dCD4-gp120 interaction as measured by competition ELISA
| Peptide | Peptide sequence | IC50a (μM)
|
||
|---|---|---|---|---|
| T-tropic gp120
|
M-tropic gp120
|
|||
| HXB2 | SF2 | ADA | ||
| 2dCD4 | 8.7 ± 2.1 nM (13) | 6.1 ± 1.6 nM (4) | 9.4 ± 0.5 nM (2) | |
| 12p1 | RINNIPWSEAMM | 25.7 ± 7.3 (13) | 0.33 ± 0.13 (6) | 4.2 ± 0.7 (2) |
| 12p1[2–8] | INNIPWS | 610 ± 130 (2) | 12 ± 3 (2) | NDb |
| 12p1[4–10] | NIPWSEA | >500 | >500 | ND |
| 12p1[6–12] | PWSEAMM | >500 | >500 | ND |
| 12p1[Scrambled] | IPAIMENMWNRS | >500 | >500 | >500 |
| 12p2 | TSPYEDWQTYLM | >500 | >500 | >500 |
| 12p3 | DTHRWPWYISQE | 500 | 259 ± 12 (2) | 348 ± 3 (2) |
| 12p5 | SPWYSDYTRYLW | >140c | >140c | ND |
| 12p6 | SDPYKLWASYMY | >100c | >100c | ND |
| 7p1 | SWPWDTW | >130c | 21 (1) | ND |
| 7p2 | FWHPMHD | >500 | 68 ± 4 (2) | >500 |
| C7Cp1 | CHPLTPWEC | >500 | >500 | >500 |
a
Values are means ± standard errors; the number of experiments is given in parentheses.
b
ND, not determined.
c
Low solubility prevented higher concentrations from being attained.