Figure 2.

Inhibition of TREM-1 attenuated TBI-ALI. A – mRNA expression of TREM-1 in lung tissue at 24, 48, and 72 h post-TBI. N = 3. B – Representative western blots of TREM-1 in lung tissue at 24, 48, and 72 h post-TBI. C – Densitometric analysis of TREM-1. N = 3. D – Immunohistochemical staining of TREM-1 at 48 h post-TBI. Scale bar = 50 μm, N = 4. E – Representative western blots of pro-inflammatory cytokines IL-6, TNF-α, and HMGB1 in lung tissue at 24, 48, and 72 h post-TBI. Densitometric analysis of (F) IL-6, (G) TNF-α, and (H) HMGB1. N = 3. I – Representative images of H&E-stained lung tissue from the sham group, TBI + NS group, and TBI + LP17 group are shown. Scale bar = 100 μm, N = 4. J – Lung injury scores based on histopathological staining of lungs. K – The wet/dry ratio of the lungs. N = 4. L – Total protein in the BALF. N = 4. M – Representative western blots of TREM-1 and NLRP3 inflammasome components. Densitometric analysis of (N) TREM-1, (O) NLRP3, (P) Cleaved caspase-1, (Q) ASC, (R) IL-1β, (S) IL-18. Results normalized to β-actin. N = 4–5. The values are presented as mean ± standard deviation (SD). *P < 0.05, **p < 0.01, ***p < 0.001 vs. Sham group. #P < 0.05, ##p < 0.01 vs. TBI + NS group