Figure 2.

Effects of Em on human keratinocytes.(a) Em cytotoxicity was assessed in vitro by MTT on HaCaT cells after 2 and 5 days of exposure. Their effect on NFAT activation was tested in vitro in human primary keratinocytes by quantifying (b) NFAT translocation after 6 hours of activation or not with P/I (bar = 20 μm) and (c) IL-8 and TNF-α production after 24 h of activation or not with P/I. Results represent the mean ± SD of 3 independent experiments. Statistical comparison was assessed using the student t-test. ∗P < .05and ∗∗P < .01. A.U., arbitrary unit; Em, pickering emulsion; h, hour; NFAT, nuclear factor of activated T cell; P/I, phorbol myristate acetate/ionomycin; PMA, phorbol myristate acetate;