Figure 4.

Effect of Em on human Langerhans cells. (a) Quantification of T-cell proliferation after 7 days of coculture with CD1a⁺ LCs isolated from epidermal cells freshly obtained from human skin and pretreated with either CsA, Tac, or Cal solutions or Em. (b) Quantification of activation markers expressed on moLCs differentiated from human blood and exposed during 48 hours to Em and TNF-α activation in culture. (c) Quantification of human blood T-cell proliferation by CFSE staining after 7 days of coculture with pretreated moLCs. Results represent the mean ± SEM of 6 independent experiments. Statistical comparison was assessed using the Wilcoxon rank-sum test. ∗P < .05 and ∗∗P < 0.01. A.U., arbitrary unit; Cal, calcitriol; CFSE, carboxyfluorescein succinimidyl ester; CsA, cyclosporin A; Em, pickering emulsion; MLR, mixed lymphocyte reaction; moLC, monocyte-derived Langerhans cell; Tac, tacrolimus.